The fluorescence signal on the right, showing the capture and derivatization of b-DNA on the beads, indicates problems that are difficult to diagnose.
BI Monitoring by Fluorescence and Absorbance
3.3.17.
Simultaneous absorbance measurement shows that the flow cell was not initially fully packed by beads, and later an air bubble moved through the bead layer. Compare with absorbance signal on the next slide when the flow cell was well packed and air was absent.
